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Cryo-electron microscopy (cryo-EM) is a technique for three-dimensional imaging of biological macromolecules. Molecules are frozen in a thin layer of ice and their tomographic projections are recorded in an electron microscope. Recent advances have yielded molecular reconstructions at near-atomic resolution, and in 2017 the Nobel Prize in Chemistry was awarded to three pioneers of the field. The goal of this minisymposium is to bring together the communities of cryo-EM and computational imaging to facilitate the exchange of new ideas and perspectives. It will cover fast algorithms for reconstruction, resolving high-resolution structures, validation, structural variability in heterogeneous samples, and other topics.