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The GcoPS method is dedicated to the co-localization of fluorescence image pairs for conventional and super-resolution microscopy. This fast procedure is controlled by a p-value and tests whether the Pearson correlation between two binary images is significantly positive. It amounts to quantifying the interaction strength by the area/volume of the intersection between the two binary images viewed as random distributions of geometrical objects. GcoPS handles 2D/3D images, variable SNRs and any kind of cell shapes.