Improvements in the DQE of direct electron detectors combined with their ability to operate in movie mode, have created unique opportunities for the development of computational strategies in single-particle cryo-EM. By harnessing the time-resolved information contained in movie frames, we developed methods to account for local variations in defocus and beam-induced drift, and implemented a data-driven dose compensation scheme that allowed us to overcome fundamental resolution barriers inherent to imaging biological samples using high-energy electrons.

This presentation is part of Minisymposium “MS51 - Algorithms for Single Particle Reconstruction in Cryo-Electron Microscopy (cryo-EM). (3 parts)”
organized by: Roy Lederman (Yale University) , Joakim Andén (Flatiron Institute) .

Authors:

Alberto Bartesaghi (CCR/NCI/NIH)

Keywords:

image enhancement, image reconstruction, image registration